科學家揭示人類卵母細胞中小極體介導的紡錘體雙極化機制

作者：小柯機器人 發布時間：2024/8/25 18:58:55

本期文章：《科學》：Volume 385 Issue 6711

復旦大學王磊等研究人員合作揭示人類卵母細胞中小極體介導的紡錘體雙極化機制。該項研究成果發表在2024年8月23日出版的《科學》雜志上。

通過在超過1800個人類卵母細胞中進行高分辨率成像，研究人員揭示了在紡錘體雙極化過程中形成的多極中間體的典型狀態，并闡明了這一過程的機制。研究人員發現，多極中間體的生成與多個動粒簇中形成的小極體有關。

研究人員進一步確定了HAUS6、KIF11和KIF18A在紡錘體雙極化中的關鍵作用，并在卵母細胞或胚胎缺陷的不育患者中發現了這些基因的突變。這些結果為人類卵母細胞中紡錘體雙極化的生理和病理機制提供了新見解。

研究人員表示，紡錘體雙極化是指微管質轉變為雙極紡錘體的過程，這是精確染色體分離的前提。與有絲分裂細胞不同，人類卵母細胞中紡錘體雙極化的過程和機制仍不清楚。

附：英文原文

Title: Mechanisms of minor pole–mediated spindle bipolarization in human oocytes

Author: Tianyu Wu, Yuxi Luo, Meiling Zhang, Biaobang Chen, Xingzhu Du, Hao Gu, Siyuan Xie, Zhiqi Pan, Ran Yu, Ruiqi Hai, Xiangli Niu, Guimin Hao, Liping Jin, Juanzi Shi, Xiaoxi Sun, Yanping Kuang, Wen Li, Qing Sang, Lei Wang

Issue&Volume: 2024-08-23

Abstract: Spindle bipolarization, the process of a microtubule mass transforming into a bipolar spindle, is a prerequisite for accurate chromosome segregation. In contrast to mitotic cells, the process and mechanism of spindle bipolarization in human oocytes remains unclear. Using high-resolution imaging in more than 1800 human oocytes, we revealed a typical state of multipolar intermediates that form during spindle bipolarization and elucidated the mechanism underlying this process. We found that the minor poles formed in multiple kinetochore clusters contribute to the generation of multipolar intermediates. We further determined the essential roles of HAUS6, KIF11, and KIF18A in spindle bipolarization and identified mutations in these genes in infertile patients characterized by oocyte or embryo defects. These results provide insights into the physiological and pathological mechanisms of spindle bipolarization in human oocytes.

DOI: ado1022

Source: https://www.science.org/doi/10.1126/science.ado1022

期刊信息

Science：《科學》，創刊于1880年。隸屬于美國科學促進會，最新IF：63.714

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